چند شکلی ریزماهواره‌ای در جمعیت‌های طبیعی گونه در معرض تهدید ماهی کلمه خزر در سواحل استان گلستان

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه شیلات، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

چکیده

به منظور بررسی تنوع ژنتیکی و ساختار جمعیتی ماهی کلمه که یکی از گونه‌های مهم اقتصادی و در معرض تهدید دریای خزر است، 58 نمونه از مناطق گرگانرود و خلیج گرگان جمع‌آوری و با استفاده از 10 جفت پرایمر ریزماهواره بررسی شد. هر 10 جایگاه ژنی مورد استفاده چند شکلی نشان دادند و دارای ظرفیت اطلاعات چند شکلی 72/0 تا 93/0 بودند. نتایج نشان داد که مناطق مورد بررسی از غنای اللی (تعداد الل:3/10) و ژنی (هتروزیگوسیتی: 67/0) قابل قبولی برخوردار هستند. در بررسی تعادل ‌هاردی- واینبرگ، 10 نمونه از 20 تست مورد بررسی (10 جایگاه ژنی × 2 منطقه) انحراف معنی‌داری از تعادل نشان دادند که علت عمده آن را می‌توان ناشی از کسری هتروزیگوسیتی مشاهده شده دانست. دندروگرام UPGMA ترسیم شده بر اساس فاصله ژنتیکی Neiنیز جدایی آشکاری را بین جمعیت‌ها نشان نداد. همچنین، آنالیز واریانس مولکولی نشان داد که تنوع بالایی (%98) در درون جمعیت‌های مورد بررسی وجود دارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Microsatellite polymorphism in natural populations of threatened Caspian roach in Golestan coasts

نویسندگان [English]

  • Hadiseh Kashiri
  • Ali Shabani
  • Bahareh Shabanpour
  • Mohammad Rezaii
Fisheries Faculty, Gorgan University of Agriculture Sciences and Natural Resources, Iran
چکیده [English]

To investigate the population structure of Caspian roach, one of the economically important and threatened species of Caspian Sea, 58 samples were collected from Gorgan river and Gorgan bay and then they were studied using 10 microsatellite loci. All of the 10 loci were polymorphic and showed polymorphism information content from 0.72 to 0.93. Results indicated that allelic (allele number: 10.3) and gene (heterozygosity: 0.67) diversity of the regions are at an acceptable level. 10 cases of 20 investigated tests (10 loci × 2 populations) showed significant deviation from Hardy-Weinberg equilibrium, mainly due to the heterozygosity deficiency. UPGMA cluster analysis, based on Nei genetic distance, didn’t show distinct departure among populations. Also, analysis of molecular variance showed high diversity (98%) within the populations.

کلیدواژه‌ها [English]

  • Primer
  • Polymorphism
  • Gorgan Bay
  • Gogran River
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