مقایسه ژنتیکی ماهی سفید دریای خزر (Kamenskii, 1901) Rutilus frisii kutum در رودخانه‌های گرگانرود و چشمه‌کیله (تنکابن) با استفاده از نشانگرهای ریزماهواره

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد گروه شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

2 استادیار گروه شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

3 دانشجوی دکتری شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

چکیده

ساختار ژنتیکی ماهی سفید سواحل جنوبی دریای خزر، با استفاده از نشانگر ریزماهواره بررسی گردید. تعداد 50 قطعه ماهی در بهار 85، از رودخانه‌های گرگانرود (استان گلستان) و چشمه کیله (استان مازندران) صید شدند. از ده جایگاه ژنی ریزماهواره‌ای، (Ca1, Ca3, CypG3, CypG24, CypG27, CypG30, Lid1, Rru2, Rru4, Z21908) در این بررسی استفاده شدند که هر ده جایگاه ژنی چند‌شکلی نشان دادند. میانگین تعداد الل در سطح جمعیت‌ها 95/7 بود که پایین‌تر از مقدار مشاهده شده در ماهیان رودکوچ است. هر دو جمعیت علایمی از بروز تنگنای ژنتیکی نشان دادند. مقدار هتروزیگوسیتی مشاهده شده در گرگانرود 80/0 و در چشمه کیله 74/0 به دست آمد. از میان 20 تست جایگاه ژنی- جمعیت در 11 تست انحراف معنی‌داری از تعادل هاردی - واینبرگ مشاهده شد. نتایج به دست آمده از Fst (008/0) و Rst (04/0) تمایز ژنتیکی پایینی را میان مناطق نشان داد. همچنین، بر اساس آنالیز واریانس مولکولی، تنها 4 درصد تنوع مشاهده شده مربوط به بین جمعیت‌هاست. میزان فاصله ژنتیکی میان مناطق نیز 03/0 به دست آمد. به نظر می-رسد مدیریت نامناسب برنامه بازسازی ذخایر ماهی سفید تأثیراتی منفی بر روی ساختار ژنتیکی این گونه گذاشته است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Genetic comparison of Caspian Sea Rutilus frisii kutum (Kamenskii, 1901) in Gorganroud and Cheshmekile (Tonekabon) rivers using microsatellite markers

نویسندگان [English]

  • Mohammad Rezaei 1
  • Ali Shabani 2
  • Bahare Shabanpour 2
  • Hadis Kashiri 3
1 Gorgan University of Agriculture Science and Natural Resources, Iran
2 Gorgan University of Agriculture Science and Natural Resources, Iran
3 Gorgan University of Agriculture Science and Natural Resources, Iran
چکیده [English]

The population structure of Rutilus frisii kutum in southern coasts of Caspian Sea was investigated using microsatellite loci. 50 Kutum fish samples were collected from Gorganroud (Golestan province) and Cheshmekile (Mazandaran province) rivers in April 2006. All the 10 loci investigated in this study (Ca1, Ca3, CypG3, CypG24, CypG27, CypG30, Lid1, Rru2, Rru4, Z21908) were polymorphic. The mean number of alleles at the population level was 7.95 which is lower than the reported values for anadromous fishes. Both populations showed signs of bottleneck. The mean value of observed heterozygosity was 0.80 for Gorganroud and 0.74 for Cheshmekile, respectively. Among the 20 tests of population-loci, 11 samples showed significant deviation from Hardy-Weinberg equilibrium. Results from Fst (0.008) and Rst (0.04) values showed low genetic differentiation among the populations, also, AMOVA analysis indicated only 4% genetic diversity between populations. The values of genetic distance was 0.03 among the regions. It seems that mismanagement of kutum’restocking programs has had negative effects on the genetic structure of this species.

کلیدواژه‌ها [English]

  • Genetic Diversity
  • Caspian Sea
  • Microsatellite
  • Genetic Structure
  • Rutilus frisii kutum

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