A Karyological Study of F1 Hybrids between Female Asp and Male Kutum (Leusiscus Aspius × Rutilus Kutum)

Document Type : Original Article

Authors

1 Ph. D, Department of Fisheries, Faculty of Marine Science, Tarbiat Modares University, Noor, Iran

2 Professor, Department of Fisheries, Faculty of Marine Science, Tarbiat Modares University, Noor, Iran

3 Professor, Department of Fisheries, Faculty of Natural resources, University of Guilan, Someasara, Iran

4 Ph. D, Department of Fisheries, Faculty of Natural resources, University of Guilan, Someasara, Iran

5 Ms. Dr. Yousefpour Marine Fishes Restocking and Genetic Conservation Center, Siahkal, Guilan, Iran

Abstract

Abstract
In this study, cytogenetic characteristics of F1 progeny produced by crossbreeding of female Asp and male Kutum (Leusiscus aspius × Rutilus frisii) were determined using the tissue-squashing method of kidney and giemsa staining. Nucleolus organizer regions were also localized using AgNO3. Chromosome number was determined from a total of 85 metaphases plates achieved from 20 hybrid specimens with an average weight of 49.7 ± 2.5 g (S.E). Karyogram and ideogram were arranged from the best metaphase plates. Different characteristics of karyotype including the number of chromosome arms, the length of short and long arms, the centromic index, the arm ratio, and the relative and total length of chromosomes were calculated. The number of diploid chromosomes and chromosome arms was 2n=50 and NF=80, respectively. The karyotypes consisted of 10 pairs of metacentric, 5 pairs of submetacentric, and 10 pairs of acrocentric to sub-telocentric (10 m+5 sm+10 a-t). The ranges of centromic index, arm ratio, and relative length were 0-0.443, 1.266-∞ and 2.265-6.554 μm, respectively. The total length of chromosomes in the haploid series was 29.98 μm. The longest and shortest chromosomes were a pair of submetacentric and telocentric chromosomes, respectively. Nucleolus organizer regions were localized on the ends of long arms of the longest pair of acro-centric. These findings confirmed that F1 hybrids inherited the number and type of chromosomes from their parents. 

Keywords

Main Subjects

References

Abdoli, A. (2008) The inland water fishes of Iran. Iran darabad wildlife and nature museum, Tehran (In Persian)
Almeida-Toledo, L. F., Bernardino, G., Oliveira, C., Foresti, F., & Toledo-Filho, A. S. (1996). Gynogenetic Fish Produced by a Backcross involving a Male Hybrid (Female Colossoma Macropomum × male Piaractus Mesopotamicus) and a Female Piaractus Mesopotamicus. Boletim Técnico do CEPTA, 9, 31-37.
Almeida-Toledo, L. F., Bigoni, A. P. V., Bernardino, G., & Toledo-Filho, S. A. (1995). Chromosomal Location of NORs and C Bands in F1 Hybrids of Bighead Carp and Silver Carp Reared in Brazil. Aquaculture Journal, 135, 277-284.
Amini, F., Zamini, A. A., & Ahmadi, M. R. (2007). Intergeneric Hybridization Between Kutum,
Rutilus Frisii Kutum, and Bream, Abramis Brama Orientalis of the Caspian Sea. Journal of the World Aquaculture Society, 38, 497-505 (in Persian).
Aminur Rahman, M., Arshad, A., Marimuthu, K., Ara, R., & Amin, S. M. N. (2013). Inter-specific Hybridization and its Potential for Aquaculture of Fin Fishes. Asian Journal of Animal and Veterinary Advances, 8, 139-153. 
Aminur Rahman, M., Lee, S., Yusoff, F., & Rafiquzzaman, S. M. (2018). Hybridization and Its Application in Aquaculture. Sex Control in Aquaculture PP, 163-178. 
Bagheri, M., Falahatkar, B., & Efatpanah, I. (2017). Growth Performance and Body Composition of Aspikutum: A New Hybrid of Asp, Leuciscus aspius ♀, × Caspian Kutum, Rutilus Frisii ♂, Cultured Under Different Stocking Densities in Circular Concrete Tanks. Journal of the World Aquaculture Society, 48, 947-954.
Bartley, D. M., Rana, K., & Immink, A. J. (2001). The Use of Inter-specific Hybrids in Aquaculture and Fisheries. Reviews in Fish Biology and Fisheries Journal, 10, 325-337.
Britton-Davidian, J., Cazaux, B., & Catalan, J. (2012). Chromosomal Dynamics of nuclear Organizer Regions (NORs) in the House Mouse: Micro-Evolutionary Insights. Heredity Journal, 108, 68-74.
Cleiton, F., & Giuliano-Caetano, L. (2008). Cytogenetic Characterization of Two Turtle Species: Trachemys Dorbigni and Trachemys Scripta Elegans. Caryologia Journal, 61, 253-257.
Coad, B. W. (2020). Freshwater Fishes of Iran. Available Online: www. Briancoad.com. Accessed 22 March 2020.
Dorafshan, S., & Kalbassi, M. R. (2007). A Karyological Study of Ctenopharyngodon Idella ♀ × Hypophtalmichthys Nobillis ♂ F1 Hybrids. Iranian Journal of Biology, 20, 277-285 (in Persian).
Dotti do Prado, F., Leite Nunes, T., Augusto Senhorini, J., Bortolozzi, J., Foresti, F., & Porto-Foresti, F. (2012). Cytogenetic Characterization of F1, F2 and Backcross Hybrids of the Neotropical Catfish Species Pseudoplatystoma Corruscans and Pseudoplatystoma Reticulatum (Pimelodidae, Siluriformes). Genetics and Molecular Biology Journal, 35(1), 57-64. 
Falahatkar, B., & Tolouei Gilani, M. H. (2013). The Use of Laparoscopy for Sex Identification of Asp Aspius aspius. North American Journal of Fisheries Management, 33, 894-899.
Falahatkar, B., Meknatkhah, B., & Efatpanah, I. (2015). Potential of Aspikutum (a hybrid of Leuciscus aspius ♀ × Rutilus frisii ♂) for Aquaculture: A Comparison of Growth Performance in Earthen Ponds and Tanks. Iranian Journal of Ichthyology, 2, 194-196 (in Persian).
Howell, W. M., & Black, D. A. (1980). Controlled Silver-staining of Nucleolus Organizer Regions with a Protective Colloidal Developer: A 1-Step Method. Experientia Journal, 36, 1014-1015.
Kalbassi, M. R., Dorafshan, S., Tavakolian, T., Khazab, M., & Abdolhay, H. (2006). Karyological Analysis of Endangered Caspian Salmon, Salmo Trutta Caspius (Kessler, 1877). Aquaculture Research Journal, 37, 1341-1347 (in Persian).
Kashefi, P., Bani, A., & Ebrahimi, E. (2012). Morphometric and Meristic Variations Between Nonreproductive and Reproductive Kutum Females (Rutilus Frisii Kutum, Kamensky, 1901), in the Southwest Caspian Sea. Italian Journal of Zoology, 79, 337-343 (in Persian).
Kennedy, B. M., Baumsteiger, J., Gale, W. L., Ardren, W. R., & Ostrand, K. G. (2009). Morphological, Physiological, and Genetic Techniques for Improving Field Identification of Steelhead, Coastal Cutthroat Trout, and Hybrid Smolts. Marine and Coastal Fisheries Journal, 1, 45-56. 
Kottelat, M., & Freyhof, J. (2007). Handbook of European Freshwater Fishes. Berlin: Publications Kottelat, Cornol and Freyhof.
Levan, A., Fredga, K., & Sandberg, A. A. (1964). Nomenclature for Centromic Position on Chromosomes. Hereditas Journal, 52, 201-220.
Lissa, S. A., Lamer, J. T., Sassd, G. G., & Suski, C. D. (2016). Physiological Consequences of Hybridization: Early Generation Backcrossing Decreases Performance in Invasive Bigheaded Carps. Journal of Freshwater Ecology, 31, 543-554.
Martyniak, A., & Heese, T. (1994). Growth Rate and Age Composition of asp Aspius (L., 1758) from Pierzcha"y Reservoir. Acta Ichthyologica et Piscatoria, 24, 55-67. 
Meknatkhah, B., Haghparast, P., Rahmati, M., Bagheri, M., & Falahatkar, B. (2016). Morphometric and Meristic Characteristics of Female Asp × male Kutum (Leuciscus Aspius ♀ × Rutilus Frisii ♂) Hybrid. Iranian Aquaculture Society Journal, 4, 1-11 (In Persian).
Noruz Fashkhami, M. R., & Khosroshahi, M. (1995). A Karyology (Chromosomal) Study of the Caspian Sea Kutum Roach by White Blood Cell Culture. Iranian Scientific Fisheries Journal, 4, 64-71 (in Persian).
Noruz Fashkhami, M. R., Pour Kazemi, M., & Kalbassi, M. R. (2001). Progeny Karyotyping of Female Rutilus Frisii Kutum × Male Ctenophartngodon Idella Hybrid. Iranian Marine Sciences and Technology Journal, 1, 69-74 (in Persian).
Oliveira, C., Foresti, F., & Hilsdorf, A. W. S. (2009). Genetics of Neotropical Fish: From Chromosomes to Populations. Fish Physiology and Biochemistry Journal, 35, 81-100.
Porto-Foresti, F., Hashimoto, D. T., Alves, A. L., Almeida, R. B. C., Senhorini, J. A., Bortolozzi, J., & Foresti, F. (2008). Cytogenetic Markers as Diagnoses in the Identification of the Hybrid Between Piauçu (Leporinus Macrocephalus) and Piapara (Leporinus Elongatus). Genetic and Molecular Biology Journal, 31, 195-202.
Rab, P., Roth, P., & Arefjev, V. A. (1990). Chromosome Studies of European Leuciscine Fishes (Pisces, Cyprini Dae). Karyotype of Aspius Aspius. Caryologia Journal, 43, 249-256.
Rezaei, E., Vatandoust, S., Kazemian, M., & Anvarifar, H. (2012). Morphological variability of the Aspius aspius taeniatus (Eichwald, 1831) in the Southern Caspian Sea Basin. Iranian Journal of Fisheries Sciences, 11, 627-643.
Rostamipour, S., Falahatkar, B., & Efatpanah, I. (2017). Determination of Appropriate Dietary Protein Level of “Aspikutum”, A Hybrid of Asp (Aspius aspius ♀) and Caspian Kutum (Rutilus frisii ♂). Journal of Aquatic Animal Nutrition, 2, 11-22 (in Persian).
Taxonomy and Biosystematics
Volume 12, Issue 42
March 2020
Pages 39-50

Files

Share

How to cite

Statistics