University of Isfahan Taxonomy and Biosystematics 3115-9001 18 69 2026 03 01 69 30747 EN Journal Article 2026 08 31 https://tbj.ui.ac.ir/article_30747_29f90557f87a19b19f9d3b5d77ef848e.pdf

University of Isfahan Taxonomy and Biosystematics 3115-9001 18 69 2026 03 01 DNA barcoding of cowries (Gastropoda: Cypraeidae) from edge of the Wallace Line, Indonesia 1 12 30313 10.22108/tbj.2026.147756.1328 EN Edwin Jefri Doctoral Program of Agricultural Science, Hasanuddin University, Makassar, Indonesia Marine Science Study Program, Faculty of Agriculture, University of Mataram, Mataram, Indonesia Jamaluddin Jompa Department of Marine Science, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia Nadiarti Nurdin Kadir Department of Fisheries, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia Aquatic Macrofaunal Biodiversity and Conservation Research Group, Hasanuddin University Nurliah Buhari Marine Science Study Program, Faculty of Agriculture, University of Mataram, Mataram, Indonesia Journal Article 2025 12 13 The intertidal zones of Lombok Island, located at the edge of the Wallace Line, represent a biogeographically important transition region that supports high levels of marine biodiversity. This study applied DNA barcoding based on the mitochondrial cytochrome c oxidase subunit I (COI) gene to identify and analyze the phylogenetic relationships of cowries (Gastropoda: Cypraeidae) collected from three coastal localities (Kayangan, Sambelia, and Sekotong). A total of seven cowrie species were confirmed, namely Bistolida hirundo (Linnaeus, 1758), Erronea errones (Linnaeus, 1758), Mauritia arabica (Linnaeus, 1758), Monetaria annulus (Linnaeus, 1758), M. moneta (Linnaeus, 1758), Naria boivinii (Kiener, 1844), and N. erosa (Linnaeus, 1758). In addition, one species from the family Ovulidae, Calpurnus verrucosus (Linnaeus, 1758), was included as the outgroup. BLAST analyses revealed high similarity values (>98%) with reference sequences in GenBank, validating the reliability of species-level identification. Genetic distance analysis using the Kimura 2-parameter (K2P) model showed clear interspecific divergence (0.114–0.232), supporting the presence of a “barcode gap” among examined taxa. Phylogenetic reconstruction using the Maximum Likelihood method showed bootstrap support generally exceeding 90%, indicating low interpopulation genetic differentiation and highlighting effective larval dispersal across oceanic regions. Nucleotide composition analysis further confirmed a consistent AT-rich bias (61–63%) typical of gastropod mitochondrial genomes, with subtle interspecific variation potentially useful for molecular diagnostics. This research provides the first DNA barcoding and phylogenetic assessment of cowries from Lombok, enriching the genetic database of Cypraeidae and offering essential baseline data for conservation, biogeographic, and evolutionary studies in the Indo-Pacific marine hotspot.

https://tbj.ui.ac.ir/article_30313_821c1bece0931bcb8773e080e54e1688.pdf

University of Isfahan Taxonomy and Biosystematics 3115-9001 18 69 2026 03 01 Assessment of leaf stomatal morphological diversity in grape cultivars (Vitis vinifera L.) and introduction of cultivars adapted to semi-arid conditions of Qazvin, Iran 13 34 30262 10.22108/tbj.2026.147177.1323 EN Sharif Rahmani Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran Fatemeh Hajmoradi Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran Gholamreza Bakhshi Khaniki Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran Journal Article 2025 10 23 Leaf stomatal morphology is a key determinant of plant adaptation to environmental stress, particularly drought. This study conducted a comprehensive assessment of stomatal traits across 26 grape cultivars (Vitis vinifera L.) cultivated in the semi-arid region of Qazvin, Iran. Over two growing seasons (2022–2023), epidermal impressions were prepared using the nail polish technique, and parameters including stomatal density, dimensions, complex type, surface coefficient, shape coefficient, and percentage of occupied area were quantified using light microscopy and image analysis software. Multivariate analyses (MANOVA, PCA) and univariate tests (ANOVA, FDR-corrected correlations) were employed. Results revealed considerable diversity in all measured traits. Cultivar 'Ahmadi' exhibited the largest stomata (polar length: 41.29 µm, equatorial width: 29.25 µm) and the lowest density (39 stomata per unit area), whereas 'Karreh Ruye' showed the highest density (124 stomata). PCA identified two independent axes of variation: stomatal size (explaining 69.6% of variance) and density-coverage (17.7%). Although an inverse trend between size and density was observed, the correlation was not statistically significant after FDR correction. Cluster analysis grouped cultivars into two main clusters, reflecting distinct morphological strategies: large stomata with low density versus small stomata with high density. The predominant stomatal complex type was anomocytic. The findings indicate that stomatal traits, particularly the size-density combination, could serve as preliminary morphological markers for screening cultivars with putative drought adaptation. Cultivars such as 'Ahmadi', which exhibit a morphology often associated with water conservation strategies, warrant further physiological evaluation under water-limited conditions.

https://tbj.ui.ac.ir/article_30262_6e7f242a847c4f99895dbab1649c2d7d.pdf

University of Isfahan Taxonomy and Biosystematics 3115-9001 18 69 2026 03 01 Psephellus gilanicus subsp. qazvinicus (Asteraceae, Cardueae), a new subspecies from Qazvin province, NW Iran 35 44 30315 10.22108/tbj.2026.148103.1332 EN Kazem Negaresh Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran Fatemeh Borna Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran Journal Article 2026 01 14 Psephellus, a genus of the tribe Cardueae in the family Asteraceae, comprises 114 currently accepted species. In Iran, the genus includes 14 species in eight sections, of which seven are endemic. For the taxonomic study of Psephellus sect. Psephelloidei (Boiss.) Wagenitz & Hellwig, extensive collections were conducted across its distribution area in northwestern Iran. Plant specimens were collected using conventional methods and identified based on reliable taxonomic sources. Moreover, numerous specimens deposited in different herbaria were examined. As a result, Psephellus gilanicus (Bornm.) Wagenitz subsp. qazvinicus Negaresh, a new subspecies of P. sect. Psephelloidei from Qazvin province, northwestern Iran, is described and illustrated. It resembles P. gilanicus subsp. gilanicus but differs from it in having a few-stemmed habit, the whole plant usually grayish, undivided median cauline leaves, straw-coloured or whitish-pale brown median and inner appendages, white flowers, and oblanceolate achenes ca. 7 × 3–3.5 mm, slightly covered with short hairs. The new taxon is notably characterized from other members of P. sect. Psephelloidei by its white flowers. In addition, the geographical distribution of the new subspecies and the closely related taxon is presented and mapped.

https://tbj.ui.ac.ir/article_30315_75d51a4dcce2a2eb5702420dd8afc199.pdf

University of Isfahan Taxonomy and Biosystematics 3115-9001 18 69 2026 03 01 Genetic and morphological trait structure in barley genotypes under rainfed conditions 45 54 30321 10.22108/tbj.2026.148026.1330 EN Naser Sabaghnia Department of Plant Genetics, Faculty of Agriculture, University of Maragheh, Iran Mohsen Janmohammadi Department of Plant Genetics, Faculty of Agriculture, University of Maragheh, Iran Asghar Ebadi Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources Moghan, University of Mohaghegh Ardabili, Ardabil, Iran Mehdi Mohebodini Department of Horticultural Science, Faculty of Agricultural Science and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran Journal Article 2026 01 04 Current research investigated twenty barley genotypes under rainfed conditions to examine variability in yield, its components, and morphological traits, while identifying key selection criteria. Biomass-related traits, such as biological yield and straw weight, displayed high variability, whereas thousand-seed weight showed minimal variation. Traits such as seed yield per plant, seed number per plant, and tiller number per plant exhibited moderate variability, highlighting their potential contribution to yield differences. Phenotypic correlation analysis revealed that yield was strongly linked to biomass yield and thousand-seed weight, suggesting that both source and sink components play essential roles in yield formation. Genotypic correlations were generally weaker than phenotypic correlations, reflecting the influence of environmental factors. However, thousand-seed weight, seed number per plant, and fertile tiller number consistently showed positive associations with yield, indicating their genetic stability and value for indirect selection. Factor analysis identified five independent factors. The first factor included biomass yield, straw weight, and seed yield, whereas the second factor represented seed yield per plant and seed number per plant. The third factor identified low-tillering genotypes, while the fourth factor reflected smaller seed size. Finally, the fifth factor captured variation in plant height. These factors emphasize the multifaceted nature of yield determination, with distinct separations between vegetative growth, reproductive efficiency, and plant size. Based on these findings, genetic improvement for seed yield under rainfed conditions should prioritize thousand-seed weight, seed number per plant, and fertile tiller number, while optimizing plant height.

https://tbj.ui.ac.ir/article_30321_8083dfc5c6351e5c403a458f2d3b930d.pdf