University of Isfahan Taxonomy and Biosystematics 3115-9001 13 48 2021 09 23 Anatomy and Histology of the Male Reproductive System Steppe Agama, Trapelus agilis (Sauria: Agamidae) مطالعۀ آناتومی و بافت‌شناسی دستگاه تولید مثلی نر آگامای استپی، Trapelus agilis (سوسماران: آگامیده) 47 58 26444 10.22108/tbj.2022.131253.1185 EN Nasrin Darabitabar MSc, Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran Rasoul Karamiani Assistant Professor of Biology, Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran Ahmad Gharzi Associate Professor, Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran Journal Article 2021 11 02 This study compared the anatomical and histological structure of the male reproductive system in <em>Trapelus agilis</em> of Agamidae. Male specimens of <em>Trapleus agilis </em>were collected in two seasons of spring and summer from Shadegan county (South Khuzestan Province). The samples were dissected in the laboratory after complete anesthesia with chloroform and die. Then, the male reproductive system was studied anatomically and then were removed from the animal's body and tissue passage steps (dehydration, clarification, infiltration, and embedding) were performed. Following the cutting stage with a rotating microtome, serial sections were prepared from the male reproductive system. The prepared slides were stained by the hematoxylin-eosin method and were examined histologically by light microscopy. Histological results showed that the testicles became larger in the reproductive phase (spring) and their lumen was full of sperm. Also in June, a large number of spermatozoa were seen in the lumen of the seminiferous tubules, while from spring to late summer, the diameter of the seminiferous tubules and the thickness of the germinal layer decreased. In late summer (September), when sexual activity was declining, the testicles were small and wrinkled, and the lumen of sperm-producing cells was almost empty of sperm, and the animal increased its body fat storage by feeding and preparing for hibernation. https://tbj.ui.ac.ir/article_26444_c6de87c62ce7170286d16f6b8030fe06.pdf