University of Isfahan Taxonomy and Biosystematics 3115-9001 5 14 2013 05 22 Isolation and identification of poly-extremophilic alkalophilic, halophilic and halotolerant bacteria from alkaline thalassohaline Gomishan wetland جداسازی و شناسایی باکتری‌های پلی‌اکستریموفیل قلیادوست، نمک‌دوست و تحمل‌کننده نمک تالاب قلیایی شور-دریایی گمیشان 79 100 17467 EN Azadeh Shahinpei Department of Microbiology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran & Microorganisms Bank, Iranian Biological Resource Centre, Karaj, Iran Mohammad Ali Amoozegar Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Sciences, University of Tehran, Tehran, Iran Microorganisms Bank, Iranian Biological Resource Centre, Karaj, Iran Abbas Akhavan-Sepahi Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran Journal Article 2016 06 14 Gomishan wetland is a natural ecosystem located in 35 km north west of Gorgan, in the west vicinity of Khajeh Nafas city and Gomishan. Twice sampling from 3 different geographic positions in dry and rainy seasons, led to the isolation of 224 isolates. For 57 isolates, halophilic and halotolerant behaviors and also optimum and growth range in different pH and temperatures were determined. Most of the moderately halophilic and halotolerant strains were capable of growing optimally in media with pH 8.5-9 and optimum growth temperatures ranging from <4 to 40 °C. The isolates were examined for hydrolytic enzymes production. Most of the isolates showed lipase activites and a total of 15, 7 and 3 strains produced amylases, proteases and DNases, respectively. The enzymes could be useful in some industrial processes. 16S rDNA phylogenetic analysis were done for 55 strains. According to this analysis, strains were placed in 22 different genera: Achromobacter, Aeromicrobium, Altererythrobacter, Bacillus, Caenispirillum, Cyclobacterium, Erythrobacter, Halobacillus, Halomonas, Idiomarina, Jonesia, Marinobacter, Martelella, Nesiotobacter, Paenibacillus, Planococcus, Pseudomonas, Rheinheimera, Saccharospirillum, Stappia, Thalassospira and Vibrio. 23% of these strains were haloalkalophilic bacteria and belonged to the Bacillus, Halobacillus, Halomonas, Idiomarina andMarinobacter.This was the first study on the culturable bacteria at Gomishan wetland, an area of considerable alkaline thalassohaline ecosystem.

https://tbj.ui.ac.ir/article_17467_0a58484c72136f78785d60e19c34f1fd.pdf