چند شکلی ریزماهواره‌ای در جمعیت‌های طبیعی گونه در معرض تهدید ماهی کلمه خزر در سواحل استان گلستان

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه شیلات، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

چکیده

به منظور بررسی تنوع ژنتیکی و ساختار جمعیتی ماهی کلمه که یکی از گونه‌های مهم اقتصادی و در معرض تهدید دریای خزر است، 58 نمونه از مناطق گرگانرود و خلیج گرگان جمع‌آوری و با استفاده از 10 جفت پرایمر ریزماهواره بررسی شد. هر 10 جایگاه ژنی مورد استفاده چند شکلی نشان دادند و دارای ظرفیت اطلاعات چند شکلی 72/0 تا 93/0 بودند. نتایج نشان داد که مناطق مورد بررسی از غنای اللی (تعداد الل:3/10) و ژنی (هتروزیگوسیتی: 67/0) قابل قبولی برخوردار هستند. در بررسی تعادل ‌هاردی- واینبرگ، 10 نمونه از 20 تست مورد بررسی (10 جایگاه ژنی × 2 منطقه) انحراف معنی‌داری از تعادل نشان دادند که علت عمده آن را می‌توان ناشی از کسری هتروزیگوسیتی مشاهده شده دانست. دندروگرام UPGMA ترسیم شده بر اساس فاصله ژنتیکی Neiنیز جدایی آشکاری را بین جمعیت‌ها نشان نداد. همچنین، آنالیز واریانس مولکولی نشان داد که تنوع بالایی (%98) در درون جمعیت‌های مورد بررسی وجود دارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Microsatellite polymorphism in natural populations of threatened Caspian roach in Golestan coasts

نویسندگان [English]

  • Hadiseh Kashiri
  • Ali Shabani
  • Bahareh Shabanpour
  • Mohammad Rezaii
Fisheries Faculty, Gorgan University of Agriculture Sciences and Natural Resources, Iran
چکیده [English]

To investigate the population structure of Caspian roach, one of the economically important and threatened species of Caspian Sea, 58 samples were collected from Gorgan river and Gorgan bay and then they were studied using 10 microsatellite loci. All of the 10 loci were polymorphic and showed polymorphism information content from 0.72 to 0.93. Results indicated that allelic (allele number: 10.3) and gene (heterozygosity: 0.67) diversity of the regions are at an acceptable level. 10 cases of 20 investigated tests (10 loci × 2 populations) showed significant deviation from Hardy-Weinberg equilibrium, mainly due to the heterozygosity deficiency. UPGMA cluster analysis, based on Nei genetic distance, didn’t show distinct departure among populations. Also, analysis of molecular variance showed high diversity (98%) within the populations.

کلیدواژه‌ها [English]

  • Primer
  • Polymorphism
  • Gorgan Bay
  • Gogran River

Angers, B. and Bernatchez, L. (1998) Combined use of SMM and non SMM methods to infer fine structure and evolutionary history of brook charr (Salvelinus fontinalis, Salmonidae) populations from microsatellites. Molecular Biology Evolution 15: 143-159.

Baerwald, M. R. and May, B. (2004) Characterization of microsatellite loci for five members of the minnow family Cyprinidae found in the Sacramento-San Joaquin Delta and its tributaries. Molecular Ecology Notes 4: 385-390.

Barinova, A., Yadrenkina, E., Nakajima, M. and Taniguchi, N. (2004) Identification and characterization of microsatellite DNA markers developed in ide Leuciscus idus and Siberian roach Rutilus rutilus. Molecular Ecology Notes 4: 86-88.

Bassam, B. J., Caetano-Anolles, G. and Gresshoff, G. M. (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Animal Biochemistry 84: 680-683.

Cross, T. F., Galvin, P. and Mcginnitty, P. (1998) Genetic considerations in stocking of Atlantic salmon, Salmo salar. In: Stocking and Introduction of Fish (ed. Cowx, I. G.). Hull International Fisheries Institute 355-370.

Dewoody, J. A. and Avise, J. C. (2000) Microsatellite variation in Marin, freshwater and anadromous fishes compare with other animal. Journal of Fish Biology 56: 461-473.

Dimsoski, P., Toth, G. P. and Bagley, M. J. (2000) Microsatellite characterization in central stoneroller Campostoma anomalum (Pisces: Cyprinidae). Molecular Ecology 9: 2187-2189.

Dixon, T. J., Coman, G. J., Arnold, S. J., Sellars, M. J., Lyons, R. E., Dierens, D., Preston, N. P. and Li, Y. (2008) Shifts in genetic diversity during domestication of Black Tiger shrimp, Penaeus monodon, monitored using two multiplexed microsatellite systems. Aquaculture 283: 1-6.

Diz, P. A. and Presa, P. (2009) The genetic diversity pattern of Mytilus alloprovincialis in Galician Rías (NW Iberian estuaries). Aquaculture 287: 278-285.

Dunham, R. A. (2004) Aquaculture and fisheries biotechnology genetic approaches. Canadian Association of Business Incubation Publishing, London.

Goudet, J. (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3).

Grassi, F., Imazio, S., Gomarasca, S., Citterio, S., Aina, R., Sgorbati, S., Sala, F., Patrignani, G. and Labra, M. (2004) Population structure and genetic variation within Valeriana wallrothii Kreyer in relation to different ecological locations. Plant Science 166: 1437-1441.

Hamilton, P. B. and Tylor, C. R. (2007) Identification of microsatellite loci for parentage analysis in roach (Rutilus rutilus) and eight other cyprinid fish by cross-species amplification and a novel test for detecting hybrids between roach and other cyprinids. Molecular Ecology Resources 8(2): 462-465.

Hansen, M. M., Ruzzante, D. E., Nielsen, E. E. and Mensberg, K. D. (2000) Microsatellite and mitochondrial DNA polymorphism reveals life history dependent interbreeding between hatchery and wild brown trout (Salmo trutta L.). Molecular Ecology 9: 583-594.

Hillis, D. M., Mortiz, C. and Mable, B. (1996) Molecular systematics. 2nd ed., Sinauer Associates, Sunderland.

Kiabi, B. H., Abdoli, A. and Naderi, M. (1999) Status of the fish fauna in the South Caspian basin of Iran. Journal of Zoology in the Middle East 18: 57-65.

Lind, C. U., Evans, B. S., Knauer, J., Taylor, J. J. U. and Jerry, D. R. (2009) Decreased genetic diversity and a reduced effective population size in cultured silver-lipped pearl oysters (Pinctada maxima). Aquaculture 286: 12-19.

Liu, Z. (2007) Aquaculture Genome Technologies. Blackwell Publishing, Oxford.

Lucentini, L., Palomba, A., Lancioni, H., Gigliarelli, L., Natali, M. and Panara, F. (2006) Microsatellite polymorphism in Italian populations of northern pike (Esox lucius). Fisheries Research 80: 251-262.

Machado-schiaffino, G., Depico, E. and Garcia-vazquez, E. (2007) Genetic variation losses in Atlantic salmon stocks created for supportive breeding. Aquaculture 264: 59-65.

Marshall, T. C., Slate, J., Kruuk, L. E. B. and Pemberton, J. M. (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology 7: 639-655.

Millennium Ecosystem Assessment (2005) Ecosystems and human well-being: Current state and trends, Volume 1: Findings of the conditions and trends working group of the millennium ecosystem assessment. Island Press, Washington D.C.

Nelson J. (1994) Fishes of the World. 3rd ed., John Wiley and Sons, NewYork.

Norris, A. T., Bradley, D. G. and Cunningham, E. P. (1999) Microsatellite genetic variation between and within farmed and wild Atlantic salmon (Salmo salar) populations. Aquaculture 180: 247-264.

Peakall, R. and Smouse, P. E. (2006) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288-295.

Pinera, J. A., Blanco, G., Vázquez, E. and Sánchez, J. A. (2007) Genetic diversity of blackspot seabream (Pagellus bogaraveo) populations in Spanish Coasts: a preliminary study. Marine Biology 151:2153-2158.

Rice, W. R. (1989) Analyzing tables of statistical tests. Evolution 43: 223-225.

Rodzen, J. A. and May, B. (2002) Inheritance of microsatellite loci in the polyploidy white sturgeon (Acipenser transmontanus). Genome 54: 1064-1076.

Slatkin, M. (1995) A measure of population subdivision based on microsatellite allele frequencies. Genetics 139:457-462.

Utter, F. M. (1991) Biochemical genetics and fishery management: an historical perspective. Journal of Fish Biology 39: 1-20.

Van Oosterhout, C., Hutchinson, W. F., Wills, D. P. M. and Shipley, P. (2004) MICROCHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4: 535-538.

Wang, C., Yu, X. and Tong, J. (2007) Microsatellite diversity and population genetic structure of redfin culter (Culter erythropterus) in fragmented lakes of the Yangtze River. Hydrobiologia 586: 321-329. 

Yeh, F. C., Yang, R. C. and Boyle, T. (1999) POPGENE version 1.3.1. Microsoft Window-bases Freeware for population Genetic Analysis. University of Alberta, Canada.

Xu, Z., Primavera, J. H., De la Pena, L. D., Pettit, P., Belak, J. and Warren, A. A. (2001) Genetic diversity of wild and cultured black tiger shrimp (Penaeus monodon) in the Philippines using microsatellites. Aquaculture 199: 13-40.

Zar, J. H. (1999) Biostatistical analysis, 4th ed., Prentice Hall Inc., Upper Saddle River, New Jersey.